Chlamydomonas PKD2 organizes mastigonemes, hair-like glycoprotein polymers on cilia
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چکیده
منابع مشابه
Function and dynamics of PKD2 in Chlamydomonas reinhardtii flagella
To analyze the function of ciliary polycystic kidney disease 2 (PKD2) and its relationship to intraflagellar transport (IFT), we cloned the gene encoding Chlamydomonas reinhardtii PKD2 (CrPKD2), a protein with the characteristics of PKD2 family members. Three forms of this protein (210, 120, and 90 kD) were detected in whole cells; the two smaller forms are cleavage products of the 210-kD prote...
متن کاملAssembly and function of Chlamydomonas flagellar mastigonemes as probed with a monoclonal antibody.
Mastigonemes are hair-like projections on the flagella of various kinds of lower eukaryotes. We obtained a monoclonal antibody (mAb-MAST1) to mastigonemes of Chlamydomonas reinhardtii, and found that it reacts with a single flagellar glycoprotein of about 230 kDa. Interestingly, immunofluorescence microscopy demonstrated that mAb-MAST1 recognizes not only the flagellar mastigonemes but also a r...
متن کاملUpdate on Chlamydomonas reinhardtii Assembly and Motility of Eukaryotic Cilia and Flagella. Lessons from Chlamydomonas reinhardtii
Cilia and flagella are among the most ancient cellular organelles, providing motility for primitive eukaryotic cells living in an aqueous environment. During adaptation to life on land, some groups of organisms, including advanced fungi, red algae, cellular slime molds, conifers, and angiosperms, lost the ability to assemble flagella (Raven et al., 1999). The centriole or basal body, which orga...
متن کاملPkd1l1 complexes with Pkd2 on motile cilia and functions to establish the left-right axis.
The internal organs of vertebrates show distinctive left-right asymmetry. Leftward extracellular fluid flow at the node (nodal flow), which is generated by the rotational movement of node cilia, is essential for left-right patterning in the mouse and other vertebrates. However, the identity of the pathways by which nodal flow is interpreted remains controversial as the molecular sensors of this...
متن کاملCaMK-II is a PKD2 target that promotes pronephric kidney development and stabilizes cilia.
Intracellular Ca²⁺ signals influence gastrulation, neurogenesis and organogenesis through pathways that are still being defined. One potential Ca²⁺ mediator of many of these morphogenic processes is CaMK-II, a conserved calmodulin-dependent protein kinase. Prolonged Ca²⁺ stimulation converts CaMK-II into an activated state that, in the zebrafish, is detected in the forebrain, ear and kidney. Au...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 2020
ISSN: 0021-9525,1540-8140
DOI: 10.1083/jcb.202001122